Bacterial isolates and their sensitivity profile from CSF samples – A 5 year study at a tertiary care hospital
Bacterial isolates and their sensitivity profile
DOI:
https://doi.org/10.33897/fumj.v6i2,%20Jan-Jun.100Keywords:
Cerebrospinal fluid, Hospital Acquired InfectionAbstract
ABSTRACT
Objective: The aim of the study was to evaluate the frequency of various bacteria isolated in cases of meningitis and their sensitivity profile.
Study design: Descriptive Cross sectional study
Study place & duration: Study place & duration: This study was carried out at Fauji Foundation Hospital Rawalpindi over a period of 5 years (January 2017 and December 2021).
Methodology: All CSF samples received in microbiology Lab during the study were centrifuged at 2500 r/min in the laboratory of Microbiology. The supernatant fluid was discarded. The sediment was inoculated on blood agar, Chocolate agar and MacConkey's agar and were incubated at 370o C for 24-72 hours aerobically and in 5% CO2. Cultures that yielded growth of any bacteria were further proceeded by standard microbiological methods of bacterial identification and sensitivity testing according to CLSI guide lines.
Results: A total of 2000 CSF samples were received during the study period examined. One hundred and fifty seven samples yielded growth of bacteria. Pseudomonas aeruginosa (30%) was the most frequent organism isolated from CSF from patients followed by methicillin resistant Staphylococcus aureus MRSA (13%).
Conclusion: Pseudomonas aeruginosa was the most frequent organism isolated from CSF of patients of meningitis followed by MRSA.
